Human protamine 1 gene structure and function affected by rs35576928 mutation: a bioinformatics analysis

Document Type : Research Paper

Authors

1 Department of Biotechnology, Pramukh Swami Science & H. D. Patel Arts College, Kadi, Gujarat 382517, India

2 Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Abstract

Human sperm contain most type 1 and type 2 protamines (PRM1) and (PRM2), protamines, which have about equal proportions in normal sperm. Protamines can infertile if they change structure or function. Nucleotide polymorphisms are one cause of changes in protamine structure and function. For protamine 1 the genetic variation rs35576928 is known. In. this study bioinformatics approach was used to investigate the effects of the rs35576928 mutation on protamine 1 structure and function. matics tools including RNAsnp, NetGene2, in this study several bioinformatics tools were used to investigate how the polymorphism affects RNA structure and splicing. The tools used included RNAsnp, NetGene2, and ASAP. To evaluate the effects of rs35576928 on proteins, other tools such as SNAP and ExPASy were also used. The analyzes have also shown that this polymorphism has the primary, secondary and The results of this study have also shown that this polymorphism has primary, secondary, and the results of this study have also shown that this polymorphism has primary, secondary, and results of this study have also shown that protamine 1 has the following functions: general functions. Infertile males may be affected by rs35576928 as it has destructive effects on protamine RNA and protein structure.

Graphical Abstract

Human protamine 1 gene structure and function affected by rs35576928 mutation: a bioinformatics analysis

Highlights

  • Protamine is a vital protein for sperm structure and function.
  • There are two types of protamine in the structure of human sperm, usually in the same proportion as normal sperm.
  • Protamine 1 contains several polymorphisms that can affect its structure and function.
  • The polymorphism rs35576928 is an exonic variant that can affect the structure of the RNA and protein of protamine.

Keywords

Main Subjects


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